Editing Vanilla (section)

===Tissue culture===
Tissue culture was first used as a means of creating vanilla plants during the 1980s at Tamil Nadu University. This was the part of the first project to grow ''V. planifolia'' in India. At that time, a shortage of vanilla planting stock was occurring in India. The approach was inspired by the work going on to tissue culture other flowering plants. Several methods have been proposed for vanilla tissue culture, but all of them begin from axillary buds of the vanilla vine.<ref>{{cite journal |doi=10.1007/BF01092772 |pmid=30727638 |title=In vitro multiplication of ''Vanilla planifolia'' using axillary bud explants |year=1997 |last1=George |first1=P. S. |last2=Ravishankar |first2=G. A. |journal=Plant Cell Reports |volume=16 |issue=7 |pages=490–494|s2cid=25158520 }}</ref><ref>{{cite journal |last1=Kononowicz |first1=H. |last2=Janick |first2=J. |year=1984 |title=In vitro propagation of Vanilla planifolia |journal=HortScience |volume=19 |issue=1 |pages=58–9|doi=10.21273/HORTSCI.19.1.58 |s2cid=89492752 |doi-access=free }}</ref> In vitro multiplication has also been achieved through culture of callus masses, protocorms, root tips and stem nodes.<ref>{{cite journal |vauthors=Giridhar P, Ravishankar GA |year=2004 |title=Efficient micropropagation of ''Vanilla planifolia'' Andr. under influence of thidiazuron, zeatin and coconut milk |journal=Indian Journal of Biotechnology |volume=3 |issue=1 |pages=113–118 |url=http://nopr.niscair.res.in/handle/123456789/5837 |url-status=live |archive-url=https://web.archive.org/web/20140506134353/http://nopr.niscair.res.in/handle/123456789/5837 |archive-date=6 May 2014 }}</ref> Description of any of these processes can be obtained from the references listed before, but all of them are successful in generation of new vanilla plants that first need to be grown up to a height of at least {{convert|30|cm|in|abbr=on}} before they can be planted in the field or greenhouse.<ref name="Anilkumar 2004"/>