Editing Bacterial vaginosis (section)

===Nugent score===
The [[Nugent score]] is now rarely used by physicians due to the time it takes to read the slides and requires the use of a trained microscopist.<ref name=Bennett2015/> A score of 0–10 is generated from combining three other scores.  The scores are as follows:<ref name="Nugent1991" />

* 0–3 is considered negative for BV
* 4–6 is considered intermediate
* 7+ is considered indicative of BV.

At least 10–20 high power (1000× oil immersion) fields are counted and an average determined.<ref name="pmid31077161">{{cite journal |vauthors=Kamga YM, Ngunde JP, Akoachere JK |title=Prevalence of bacterial vaginosis and associated risk factors in pregnant women receiving antenatal care at the Kumba Health District (KHD), Cameroon |journal=BMC Pregnancy and Childbirth |volume=19 |issue=1 |pages=166 |date=May 2019 |pmid=31077161 |pmc=6511194 |doi=10.1186/s12884-019-2312-9 |doi-access=free }}</ref>

{| class="wikitable"
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Lactobacillus morphotypes – average per high powered (1000× oil immersion) field. View multiple fields.
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[[Gardnerella]] / [[Bacteroides]] morphotypes – average per high powered (1000× oil immersion) field. View multiple fields.
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Curved Gram variable rods – average per high powered (1000× oil immersion) field. View multiple fields (note that this factor is less important – scores of only 0–2 are possible)
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* Score 0 for >30
* Score 1 for 15–30
* Score 2 for 14
* Score 3 for <1 (this is an average, so results can be >0, yet <1)
* Score 4 for 0
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* Score 0 for 0
* Score 1 for <1 (this is an average, so results can be >0, yet <1)
* Score 2 for 1–4
* Score 3 for 5–30
* Score 4 for >30
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* Score 0 for 0
* Score 1 for <5
* Score 2 for 5+{{Citation needed|date=January 2016}}
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[[DNA hybridization]] testing with Affirm VPIII was compared to the Gram stain using the Nugent criteria.<ref name="pmid17078859">{{cite journal | vauthors = Gazi H, Degerli K, Kurt O, Teker A, Uyar Y, Caglar H, Kurutepe S, Surucuoglu S | display-authors = 6 | title = Use of DNA hybridization test for diagnosing bacterial vaginosis in women with symptoms suggestive of infection | journal = APMIS | volume = 114 | issue = 11 | pages = 784–787 | date = November 2006 | pmid = 17078859 | doi = 10.1111/j.1600-0463.2006.apm_485.x | s2cid = 36677972 }}</ref> The Affirm VPIII test may be used for the rapid diagnosis of BV in symptomatic women but uses expensive proprietary equipment to read results, and does not detect other pathogens that cause BV, including ''Prevotella'' spp, ''Bacteroides'' spp, and ''Mobiluncus'' spp.<ref>{{cite journal | vauthors = Brown HL, Fuller DD, Jasper LT, Davis TE, Wright JD | title = Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis | journal = Infectious Diseases in Obstetrics and Gynecology | volume = 12 | issue = 1 | pages = 17–21 | date = 2004 | pmid = 15460191 | pmc = 1784585 | doi = 10.1080/1064744042000210375 }}</ref> The cervicovaginal microbiome measured using 16S rRNA sequencing has the capacity to increase throughput of the Nugent Score and has demonstrate to be directly comparable to clinical Nugent Score measurement.<ref>{{cite journal | vauthors = Usyk M, Schlecht NF, Pickering S, Williams L, Sollecito CC, Gradissimo A, Porras C, Safaeian M, Pinto L, Herrero R, Strickler HD, Viswanathan S, Nucci-Sack A, Diaz A, Burk RD | display-authors = 6 | title = molBV reveals immune landscape of bacterial vaginosis and predicts human papillomavirus infection natural history | journal = Nature Communications | volume = 13 | issue = 1 | pages = 233 | date = January 2022 | pmid = 35017496 | pmc = 8752746 | doi = 10.1038/s41467-021-27628-3 | bibcode = 2022NatCo..13..233U }}</ref>