Editing Trypsin (section)

==Applications==
Trypsin is available in high quantity in pancreases, and can be purified rather easily. Hence, it has been used widely in various biotechnological processes.

In a [[tissue culture]] lab, trypsin is used to resuspend cells adherent to the cell culture dish wall during the process of harvesting cells.<ref>{{cite web|url=http://www.stemcell.com/en/Products/All-Products/TrypsinEDTA-025.aspx|title=Trypsin-EDTA (0.25%) |publisher=Stem Cell Technologies |access-date=2012-02-23}}</ref>   Some cell types adhere to the sides and bottom of a dish when cultivated ''[[in vitro]]''. Trypsin is used to cleave proteins holding the cultured cells to the dish, so that the cells can be removed from the plates.

Trypsin can also be used to dissociate dissected cells (for example, prior to cell fixing and sorting).

Trypsin can be used to break down [[casein]] in breast milk. If trypsin is added to a solution of milk powder, the breakdown of casein causes the milk to become [[translucent]]. The rate of reaction can be measured by using the amount of time needed for the milk to turn translucent.

Trypsin is commonly used in biological research during [[proteomics]] experiments to digest proteins into peptides for mass spectrometry analysis, e.g. [[in-gel digestion]]. Trypsin is particularly suited for this, since it has a very well defined specificity, as it hydrolyzes only the peptide bonds in which the carbonyl group is contributed either by an arginine or lysine residue.

Trypsin can also be used to dissolve blood clots in its microbial form and treat inflammation in its pancreatic form.

In veterinary medicine, trypsin is an ingredient in wound spray products, such as Debrisol, to dissolve dead tissue and pus in wounds in horses, cattle, dogs, and cats.<ref>{{Cite web | url = https://www.drugs.com/international/debrisol.html | title = Debrisol | publisher = drugs.com }}</ref>

In India, Trypsin - Chymotrypsin is widely prescribed for inflammation reduction during laryngitis and surgery recovery. Use outside India is not well documented and most papers written on its effectivity in the aforementioned situations have been funded by Torrent Pharmaceuticals which is one major brand that makes these tablets in India.<ref>{{cite journal | pmc=5778189 | date=2017 | title=The Role of Trypsin:Chymotrypsin in Tissue Repair | journal=Advances in Therapy | volume=35 | issue=1 | pages=31–42 | doi=10.1007/s12325-017-0648-y | pmid=29209994 | vauthors = Shah D, Mital K }}</ref>
<ref>https://www.carehospitals.com/medicine-detail/trypsin-chymotrypsin</ref>

=== In food ===
Commercial protease preparations usually consist of a mixture of various protease enzymes that often includes trypsin. These preparations are widely used in food processing:<ref name="urlProtease - GMO Database">{{cite web | url = http://www.gmo-compass.org/eng/database/enzymes/94.protease.html | title = Protease - GMO Database | date = 2010-07-10 | work = GMO Compass | publisher = European Union | access-date = 2012-01-01 | url-status = dead | archive-url = https://web.archive.org/web/20150224164346/http://www.gmo-compass.org/eng/database/enzymes/94.protease.html | archive-date = 2015-02-24 }}</ref>
* as a baking enzyme to improve the workability of dough
* in the extraction of seasonings and flavorings from vegetable or animal proteins and in the manufacture of sauces 
* to control aroma formation in cheese and milk products 
* to improve the texture of fish products 
* to tenderize meat 
* during cold stabilization of beer 
* in the production of [[hypoallergenic]] food where proteases break down specific [[allergen]]ic proteins into nonallergenic peptides, for example, proteases are used to produce hypoallergenic baby food from cow's milk, thereby diminishing the risk of babies developing [[milk allergy|milk allergies]].