Editing Fluorescence (section)

==== Microscopy ====
* When scanning the fluorescence intensity across a plane one has [[fluorescence microscope|fluorescence microscopy]] of tissues, cells, or subcellular structures, which is accomplished by labeling an antibody with a fluorophore and allowing the antibody to find its target antigen within the sample. Labelling multiple antibodies with different fluorophores allows visualization of multiple targets within a single image (multiple channels). DNA microarrays are a variant of this.
* Immunology: An antibody is first prepared by having a fluorescent chemical group attached, and the sites (e.g., on a microscopic specimen) where the antibody has bound can be seen, and even quantified, by the fluorescence.
* FLIM ([[Fluorescence Lifetime Imaging Microscopy]]) can be used to detect certain bio-molecular interactions that manifest themselves by influencing fluorescence lifetimes.
* Cell and molecular biology: detection of [[colocalization]] using fluorescence-labelled antibodies for selective detection of the antigens of interest using specialized software such as ImageJ.