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=== Biochemistry === Caves have an absence of stable [[temperature]], high relative [[humidity]], low rates of [[evaporation]] and limited supply of [[Organic matter|organic material]], which help in creating an environment which is highly favorable for the growth of [[microbes]]. Microbial assemblages in caves include [[archaea]], [[bacteria]], [[Fungus|fungi]] and other micro-[[Eukaryote|eukaryotes]], these highly adapted microbial communities represent the living-backbone of cave ecosystems and play a key role in shaping structures and sustaining trophic networks.<ref>{{Cite web |date=January 13, 2023 |title=Microbial diversity and proxy species for human impact in Italian karst caves |url=https://www.nature.com/articles/s41598-022-26511-5#:~:text=Microbial%20assemblages%20in%20caves%20include,networks5%2C6%2C7. |access-date=August 20, 2024 |website=scientific reports}}</ref>
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