Editing Shotgun sequencing (section)

=== Approach ===
To apply the strategy, a high-molecular-weight DNA strand is sheared into random fragments, size-selected (usually 2, 10, 50, and 150 kb), and [[clone (genetics)|clone]]d into an appropriate [[vector DNA|vector]]. The clones are then sequenced from both ends using the [[Sanger sequencing#Method|chain termination method]] yielding two short sequences. Each sequence is called an ''end-read'' or ''read 1 and read 2'' and two reads from the same clone are referred to as ''[[paired-end tag|mate pairs]]''. Since the chain termination method usually can only produce reads between 500 and 1000 bases long, in all but the smallest clones, mate pairs will rarely overlap.