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====Peronosporeae==== De Bary published his first work on potato blight fungi in 1861, and then spent more than 15 years studying Peronosporeae, particularly ''Phytophthora infestans'' (formerly ''Peronospora infestans'') and ''[[Albugo candida|Cystopus]]'' (''[[Albugo]]''), parasites of [[potato]]. In his published work in 1863 entitled "Recherches sur le developpement de quelques champignons parasites", he reported inoculating healthy potato leaves with spores of ''P. infestans''. He observed that mycelium penetrated the leaf and affected the tissue, forming [[conidia]] and the black spots characteristic of potato blight. He did similar experiments on [[tuber]]s and potato stalks. He watched conidia in the soil and their infection of the tubers, observing that [[mycelium]] could survive the cold winter in the tubers. Based on these studies, he concluded that organisms were not being generated spontaneously.<ref name="Robinson"/>
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