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== Characteristics of ''Bacillus'' spp. == S.I. Paul et al. (2021)<ref name="Paul_2021">{{cite journal | vauthors = Paul SI, Rahman MM, Salam MA, Khan MA, Islam MT |date=2021-12-15|title=Identification of marine sponge-associated bacteria of the Saint Martin's island of the Bay of Bengal emphasizing on the prevention of motile Aeromonas septicemia in Labeo rohita |journal= Aquaculture |volume=545 |pages=737156 |doi=10.1016/j.aquaculture.2021.737156 |bibcode=2021Aquac.54537156P }}</ref> isolated and identified multiple strains of ''[[Bacillus subtilis]]'' (strains WS1A,<ref>{{cite journal | url = https://www.ncbi.nlm.nih.gov/nuccore/MK910097.1/ | title = Bacillus subtilis strain WS1A | journal = GenBank | date = 19 May 2019 | publisher = U.S. National Library of Medicine }}</ref> YBS29,<ref>{{cite journal | url = https://www.ncbi.nlm.nih.gov/nuccore/MT605348.1/| title = Bacillus subtilis strain YBS29 | journal = GenBank | date = 19 June 2020 | publisher = U.S. National Library of Medicine }}</ref> KSP163A,<ref>{{cite journal | url = https://www.ncbi.nlm.nih.gov/nuccore/MK910103.1/ | title = Bacillus subtilis strain KSP163A | journal = GenBank | date = 19 May 2019 | publisher = U.S. National Library of Medicine }}</ref> OA122,<ref>{{cite journal | url = https://www.ncbi.nlm.nih.gov/nuccore/MT611945.1/ | title = Bacillus subtilis strain OA122 | journal = GenBank | date = 20 June 2020 | publisher = U.S. National Library of Medicine }}</ref> ISP161A,<ref>{{cite journal | url = https://www.ncbi.nlm.nih.gov/nuccore/MT611943.1/ | title = Bacillus subtilis strain ISP161A | journal = GenBank | date = 20 June 2020 | publisher = U.S. National Library of Medicine }}</ref> OI6,<ref>{{cite journal | url = https://www.ncbi.nlm.nih.gov/nuccore/MT605347.1/ | title = Bacillus subtilis strain OI6 | journal = GenBank | date = 19 June 2020 | publisher = U.S. National Library of Medicine }}</ref> WS11,<ref>{{cite journal | url = https://www.ncbi.nlm.nih.gov/nuccore/MK910101.1/ | title = Bacillus subtilis strain WS11 | journal = GenBank | date = 19 May 2019 | publisher = U.S. National Library of Medicine }}</ref> KSP151E,<ref>{{cite journal | url = https://www.ncbi.nlm.nih.gov/nuccore/MT605346.1/ | title = Bacillus subtilis strain KSP151E | journal = GenBank | date = 19 June 2020 | publisher = U.S. National Library of Medicine }}</ref> and S8,<ref>{{cite journal | url = https://www.ncbi.nlm.nih.gov/nuccore/MT611946.1/ | title = Bacillus subtilis strain S8 | journal = GenBank | date = 20 June 2020 | publisher = U.S. National Library of Medicine }}</ref>) from [[Sponge|marine sponges]] of the [[St. Martin's Island|Saint Martin's Island]] Area of the [[Bay of Bengal]], [[Bangladesh]]. Based on their study, colony, morphological, physiological, and biochemical characteristics of ''Bacillus'' spp. are shown in the Table below.<ref name="Paul_2021" /> {| class="wikitable" ! Test type ! Test ! Characteristics |- | rowspan="4" |Colony characters |Size |Medium |- |Type |Round |- |Color |Whitish |- |Shape |Convex |- |Morphological characters |Shape |Rod |- | rowspan="2" |Physiological characters |Motility |<nowiki>+</nowiki> |- |Growth at 6.5% NaCl |<nowiki>+</nowiki> |- | rowspan="12" |Biochemical characters |Gram's staining |<nowiki>+</nowiki> |- |Oxidase |<nowiki>-</nowiki> |- |Catalase |<nowiki>+</nowiki> |- |Oxidative-Fermentative |O/F |- |Motility |<nowiki>+</nowiki> |- |Methyl Red |<nowiki>+</nowiki> |- |Voges-Proskauer |<nowiki>-</nowiki> |- |Indole |<nowiki>-</nowiki> |- |H<sub>2</sub>S Production |<nowiki>+/β</nowiki> |- |Urease |<nowiki>-</nowiki> |- |Nitrate reductase |<nowiki>+</nowiki> |- |Ξ²-Galactosidase |<nowiki>+</nowiki> |- | rowspan="6" |Hydrolysis of |Gelatin |<nowiki>+</nowiki> |- |Aesculin |<nowiki>+</nowiki> |- |Casein |<nowiki>+</nowiki> |- |Tween 40 |<nowiki>+</nowiki> |- |Tween 60 |<nowiki>+</nowiki> |- |Tween 80 |<nowiki>+</nowiki> |- | rowspan="13" |Acid production from |Glycerol |<nowiki>+</nowiki> |- |Galactose |<nowiki>+</nowiki> |- |D-Glucose |<nowiki>+</nowiki> |- |D-Fructose |<nowiki>+</nowiki> |- |D-Mannose |<nowiki>+</nowiki> |- |Mannitol |<nowiki>+</nowiki> |- |N-Acetylglucosamine |<nowiki>+</nowiki> |- |Amygdalin |<nowiki>+</nowiki> |- |Maltose |<nowiki>+</nowiki> |- |D-Melibiose |<nowiki>+</nowiki> |- |D-Trehalose |<nowiki>+</nowiki> |- |Glycogen |<nowiki>+</nowiki> |- |D-Turanose |<nowiki>+</nowiki> |} Note: + = Positive, β =Negative, O= Oxidative, F= Fermentative
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