Editing Fluorescence (section)

=== Analytical chemistry ===
Many analytical procedures involve the use of a [[fluorometer]], usually with a single exciting wavelength and single detection wavelength. Because of the sensitivity that the method affords, fluorescent molecule concentrations as low as 1 part per trillion can be measured.<ref>{{Cite journal | doi = 10.1006/abio.1993.1020| title = Fluorometric Assay Using Dimeric Dyes for Double- and Single-Stranded DNA and RNA with Picogram Sensitivity| journal = Analytical Biochemistry| volume = 208| issue = 1| pages = 144–150| year = 1993| last1 = Rye | first1 = H. S. | last2 = Dabora | first2 = J. M. | last3 = Quesada | first3 = M. A. | last4 = Mathies | first4 = R. A. | last5 = Glazer | first5 = A. N. | pmid=7679561}}</ref>

Fluorescence in several wavelengths can be detected by an [[Chromatography detector|array detector]], to detect compounds from [[High-performance liquid chromatography|HPLC]] flow. Also, [[Thin layer chromatography|TLC]] plates can be visualized if the compounds or a coloring reagent is fluorescent. Fluorescence is most effective when there is a larger ratio of atoms at lower energy levels in a [[Boltzmann distribution]]. There is, then, a higher probability of excitement and release of photons by lower-energy atoms, making analysis more efficient.