Editing Sarin (section)

===Diagnostic tests===
Controlled studies in healthy men have shown that a nontoxic 0.43&nbsp;mg oral dose administered in several portions over a 3-day interval caused average maximum depressions of 22 and 30%, respectively, in plasma and erythrocyte acetylcholinesterase levels. A single acute 0.5&nbsp;mg dose caused mild symptoms of intoxication and an average reduction of 38% in both measures of acetylcholinesterase activity. Sarin in blood is rapidly degraded either ''in vivo'' or ''in vitro''. Its primary inactive [[metabolite]]s have ''in vivo'' serum half-lives of approximately 24 hours. The serum level of unbound isopropyl methylphosphonic acid (IMPA), a sarin [[hydrolysis]] product, ranged from 2–135&nbsp;μg/L in survivors of a terrorist attack during the first four hours post-exposure. Sarin or its metabolites may be determined in blood or urine by gas or liquid [[chromatography]], while acetylcholinesterase activity is usually measured by enzymatic methods.<ref>{{cite book |last1=Baselt |first1=Randall C. |last2=Cravey |first2=Robert H. |title=Disposition of Toxic Drugs and Chemicals in Man |publisher=Biomedical Publications |location=Seal Beach, California |date=2017 |isbn=978-0-8151-0547-3 |pages=1926–1928 |url=https://archive.org/details/dispositionoftox00base |url-access=registration}}</ref>

A newer method called "fluoride regeneration" or "fluoride reactivation" detects the presence of nerve agents for a longer period after exposure than the methods described above. Fluoride reactivation is a technique that has been explored since at least the early 2000s. This technique obviates some of the deficiencies of older procedures. Sarin not only reacts with the water in the blood plasma through hydrolysis (forming so-called 'free metabolites'), but also reacts with various proteins to form 'protein adducts'. These protein adducts are not so easily removed from the body, and remain for a longer period of time than the free metabolites. One clear advantage of this process is that the period, post-exposure, for determination of sarin exposure is much longer, possibly five to eight weeks according to at least one study.<ref>{{cite report |title=Fluoride Ion Regeneration of Sarin (GB) from Minipig Tissue and Fluids Following Whole-Body GB Vapor Exposure |date=July 2003 |publisher=United States Army |last=Jakubowski |display-authors=etal |url=https://apps.dtic.mil/sti/tr/pdf/ADA484093.pdf |access-date=March 20, 2024 |url-status=live |archive-url=https://web.archive.org/web/20160102095524/http://www.dtic.mil/dtic/tr/fulltext/u2/a484093.pdf |archive-date=January 2, 2016}}</ref><ref>{{cite journal |title=Improvements of the Fluoride Reactivation Method for the Verification of Nerve Agent Exposure |last=Degenhardt |display-authors=etal |date=July 2004 |journal=[[Journal of Analytical Toxicology]] |publisher=[[Oxford University Press]] |location=Oxfordshire, England |volume=28 |issue=5 |pages=364–371 |pmid=15239857 |doi=10.1093/jat/28.5.364 |doi-access=free}}</ref>