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==In anoxic environments== Three species of Loricifera have been found in the oxygen-free sediments at the bottom of the [[L'Atalante basin]] in [[Mediterranean Sea]], more than 3,000 meters down, the first multicellular organisms known to spend their entire lives in an anoxic environment. Initially, it was thought that they are able to do this because their [[mitochondria]] act like [[hydrogenosome]]s, allowing them to respire anaerobically.<ref name=Fang-2010-04-08-Nature/><ref name=Milius-2010-04-09-ScienceNews/> However, by 2021, questions arose as to whether or not they have mitochondria.<ref name=Snyder-2021-05-06-Axios/> The newly reported animals complete their life cycle in the total absence of light and oxygen, and they are less than a millimetre in size.<ref name=Mentel-Martin-2010/> They were collected from a deep basin at the bottom of the Mediterranean Sea, where they inhabit a nearly salt-saturated [[brine]] that, because of its density (> 1.2 g/cm<sup>3</sup>), does not mix with the waters above.<ref name=Mentel-Martin-2010/> As a consequence, this environment is completely anoxic and, due to the activity of [[sulfate]] reducers, contains sulphide at a concentration of 2.9 mM.<ref name=Mentel-Martin-2010/> Despite such harsh conditions, this anoxic and sulphidic environment is teeming with microbial life, both chemosynthetic prokaryotes that are [[primary producer]]s, and a broad diversity of eukaryotic [[heterotroph]]s at the next [[trophic level]].<ref name=Mentel-Martin-2010/>
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