Editing Lactase (section)

=== Industrial use ===
Lactase produced commercially can be extracted both from [[yeast]]s such as ''[[Kluyveromyces fragilis]]'' and ''[[Kluyveromyces lactis]]'' and from molds, such as ''[[Aspergillus niger]]'' and ''[[Aspergillus oryzae]]''.<ref>{{cite journal|vauthors=Seyis I, Aksoz N |year=2004|title=Production of Lactase by ''Trichoderma'' sp. |journal=Food Technology and Biotechnology|volume=42|issue=2|pages=121–124|url=https://hrcak.srce.hr/file/163289}}</ref> Its primary commercial use in supplements is to break down lactose in milk to make it suitable for people with lactose intolerance.<ref>{{cite web |url=https://www.fda.gov/downloads/Food/IngredientsPackagingLabeling/GRAS/NoticeInventory/UCM400718 |archive-url=https://wayback.archive-it.org/7993/20171031042344/https://www.fda.gov/downloads/Food/IngredientsPackagingLabeling/GRAS/NoticeInventory/UCM400718.pdf#page=8 |url-status=dead |title=GRAS Notification for Acid Lactase from ''Aspergillus oryzae'' Expressed in ''Aspergillus niger'' |author=DSM Food Specialties |via=U.S. Food and Drug Administration |date=3 April 2014 |archive-date=31 October 2017 |page=1}}</ref><ref>{{cite book |title=New Crops, New Uses, New Markets: 1992 Yearbook of Agriculture |last=Holsinger |first=Virginia H. |publisher=U.S. Department of Agriculture |year=1992 |pages=256–258 |chapter=Innovative Products for Food Industries: The Lactaid Story |chapter-url=https://naldc.nal.usda.gov/download/IND93048088/PDF |access-date=2022-01-11 |archive-date=2022-07-17 |archive-url=https://web.archive.org/web/20220717021856/https://naldc.nal.usda.gov/download/IND93048088/PDF |url-status=dead }}</ref> The [[U.S. Food and Drug Administration]] has not independently evaluated these products.<ref>{{cite web|url=https://www.fda.gov/Food/FoodIngredientsPackaging/GenerallyRecognizedasSafeGRAS/GRASListings/ucm153949.htm|title=Agency Response Letter GRAS Notice No. GRN 000132|author=Tarantino, LM|date=12 December 2003|publisher=U.S. Food and Drug Administration|archive-url=https://web.archive.org/web/20110326081205/https://www.fda.gov/Food/FoodIngredientsPackaging/GenerallyRecognizedasSafeGRAS/GRASListings/ucm153949.htm|archive-date=26 March 2011|url-status=dead}}</ref>

Lactase (or a similar form of [[beta-galactosidase|β-galactosidase]]) is also used to screen for [[blue white screen|blue white]] colonies in the [[multiple cloning site]]s of various [[Plasmid#Vectors|plasmid vectors]] in ''[[Escherichia coli]]'' or other bacteria.<ref>{{cite web |url=https://lactaseinbioinfo.weebly.com/introduction.html |title=Introduction |vauthors=Lau HM, Lee LS, Soh WC, Tue SW |publisher=Universiti Teknologi Malaysia |website=Lactase |date=March 2013 |access-date=16 November 2018}}</ref><ref>{{cite web |url=http://www.fermentas.com/techinfo/nucleicacids/mappbluescriptiiskks.htm |archive-url=https://web.archive.org/web/20081019115101/http://www.fermentas.com/techinfo/nucleicacids/mappbluescriptiiskks.htm |url-status=dead |title=pBluescript II KS(+/−), pBluescript II SK(+/−): description & restriction map |publisher=Fermentas |archive-date=19 October 2008}}</ref>