Editing DNA vaccine (section)

== Disadvantages ==

* Limited to protein immunogens (not useful for non-protein based antigens such as bacterial polysaccharides)
* Potential for atypical processing of bacterial and parasite proteins<ref name="Robinson2000" />
* Potential when using nasal spray administration of plasmid DNA nanoparticles to transfect non-target cells, such as brain cells<ref name="harmon14">{{cite journal | vauthors = Harmon BT, Aly AE, Padegimas L, Sesenoglu-Laird O, Cooper MJ, Waszczak BL | title = Intranasal administration of plasmid DNA nanoparticles yields successful transfection and expression of a reporter protein in rat brain | journal = Gene Therapy | volume = 21 | issue = 5 | pages = 514–521 | date = May 2014 | pmid = 24670994 | doi = 10.1038/gt.2014.28 | s2cid = 5560134 | doi-access = free }}</ref>
* Cross-contamination when manufacturing different types of live vaccines in same facility