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===Low ambient temperature=== Low ambient temperature as a physical factor affecting free radical levels was identified as a treatment producing exceptional lifespan increase in Drosophila melanogaster and other living beings.<ref>{{cite journal | vauthors = Shaposhnikov MV, Guvatova ZG, Zemskaya NV, Koval LA, Schegoleva EV, Gorbunova AA, Golubev DA, Pakshina NR, Ulyasheva NS, Solovev IA, Bobrovskikh MA, Gruntenko NE, Menshanov PN, Krasnov GS, Kudryavseva AV, Moskalev AA | display-authors = 6 | title = Molecular mechanisms of exceptional lifespan increase of Drosophila melanogaster with different genotypes after combinations of pro-longevity interventions | journal = Communications Biology | volume = 5 | issue = 1 | pages = 566 | date = June 2022 | pmid = 35681084 | pmc = 9184560 | doi = 10.1038/s42003-022-03524-4 }}</ref>
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