Editing Diatom (section)

=== Expressed sequence tagging ===
In 2002, the first insights into the properties of the ''[[Phaeodactylum tricornutum]]'' gene repertoire were described using 1,000 [[expressed sequence tag]]s (ESTs).<ref name="Scala 2002">{{cite journal |doi=10.1104/pp.010713 |pmid=12114555 |pmc=166495 |title=Genome Properties of the Diatom Phaeodactylum tricornutum |journal=Plant Physiology |volume=129 |issue=3 |pages=993–1002 |year=2002 |last1=Scala |first1=S. |last2=Carels |first2=N |last3=Falciatore |first3=A |last4=Chiusano |first4=M. L. |last5=Bowler |first5=C }}</ref> Subsequently, the number of ESTs was extended to 12,000 and the diatom EST database was constructed for functional analyses.<ref name="Maheswari 2005">{{cite journal |doi=10.1093/nar/gki121 |pmid=15608213 |pmc=540075 |title=The Diatom EST Database |journal=Nucleic Acids Research |volume=33 |issue=Database issue |pages=D344–7 |year=2004 |last1=Maheswari |first1=U. |last2=Montsant |first2=A |last3=Goll |first3=J |last4=Krishnasamy |first4=S |last5=Rajyashri |first5=K. R. |last6=Patell |first6=V. M. |last7=Bowler |first7=C. }}</ref> These sequences have been used to make a comparative analysis between ''P. tricornutum'' and the putative complete proteomes from the [[green algae|green alga]] ''[[Chlamydomonas reinhardtii]]'', the [[red alga]] ''[[Cyanidioschyzon merolae]]'', and the diatom ''[[Thalassiosira pseudonana]]''.<ref name="Montsant 2005">{{cite journal |doi=10.1104/pp.104.052829 |pmid=15665249 |pmc=1065351 |title=Comparative Genomics of the Pennate Diatom Phaeodactylum tricornutum |journal=Plant Physiology |volume=137 |issue=2 |pages=500–13 |year=2005 |last1=Montsant |first1=A. |last2=Jabbari |first2=K |last3=Maheswari |first3=U |last4=Bowler |first4=C }}</ref> The diatom EST database now consists of over 200,000 ESTs from ''P. tricornutum'' (16 libraries) and ''T. pseudonana'' (7 libraries) cells grown in a range of different conditions, many of which correspond to different abiotic stresses.<ref>{{cite journal |doi=10.1093/nar/gkn905 |pmid=19029140 |pmc=2686495 |title=Update of the Diatom EST Database: A new tool for digital transcriptomics |journal=Nucleic Acids Research |volume=37 |issue=Database issue |pages=D1001–5 |year=2009 |last1=Maheswari |first1=U. |last2=Mock |first2=T. |last3=Armbrust |first3=E. V. |last4=Bowler |first4=C. }}</ref>