Editing Optical microscope (section)

====STED====
[[File:MAX 052913 STED Phallloidin.png|thumb|right|300px|Stimulated emission depletion (STED) microscopy image of actin filaments within a cell]]
[[STED microscope|Stimulated emission depletion]] is a simple example of how higher resolution surpassing the diffraction limit is possible, but it has major limitations. STED is a fluorescence microscopy technique which uses a combination of light pulses to induce fluorescence in a small sub-population of fluorescent molecules in a sample. Each molecule produces a diffraction-limited spot of light in the image, and the centre of each of these spots corresponds to the location of the molecule. As the number of fluorescing molecules is low the spots of light are unlikely to overlap and therefore can be placed accurately. This process is then repeated many times to generate the image. [[Stefan Hell]] of the Max Planck Institute for Biophysical Chemistry was awarded the 10th German Future Prize in 2006 and Nobel Prize for Chemistry in 2014 for his development of the STED microscope and associated methodologies.<ref>{{cite web|url = http://www.heise.de/english/newsticker/news/81528|title = German Future Prize for crossing Abbe's Limit|access-date = 24 February 2009|url-status = live|archive-url = https://web.archive.org/web/20090307040808/http://www.heise.de/english/newsticker/news/81528|archive-date = 7 March 2009|df = dmy-all}}</ref>