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=== Epitope mapping === Due to their various epitopes, antibodies have gained interest in both basic and clinical research. The foundation of antibody characterization and validation is epitope mapping. The procedure of identifying an antibody's binding sites (epitopes) on the target protein is referred to as "epitope mapping." Finding the binding epitope of an antibody is essential for the discovery and creation of novel vaccines, diagnostics, and therapeutics.<ref name=":3" /> As a result, various methods for mapping antibody epitopes have been created. At this point, western blotting's specificity is the main feature that sets it apart from other epitope mapping techniques. There are several application of western blot for epitope mapping on human skin samples, hemorrhagic disease virus.<ref name=":3" /><ref>{{cite journal | vauthors = Maier RH, Maier CJ, Rid R, Hintner H, Bauer JW, Onder K | title = Epitope mapping of antibodies using a cell array-based polypeptide library | journal = Journal of Biomolecular Screening | volume = 15 | issue = 4 | pages = 418β426 | date = April 2010 | pmid = 20233905 | doi = 10.1177/1087057110363821 | s2cid = 210137 | doi-access = free }}</ref><ref>{{cite journal | vauthors = Kong D, Liu J, Jiang Q, Yu Z, Hu X, Guo D, Huang Q, Jiao M, Qu L | display-authors = 6 | title = Production, Characterization, and Epitope Mapping of Monoclonal Antibodies Against Different Subtypes of Rabbit Hemorrhagic Disease Virus (RHDV) | journal = Scientific Reports | volume = 6 | issue = 1 | pages = 20857 | date = February 2016 | pmid = 26878800 | pmc = 4754648 | doi = 10.1038/srep20857 | bibcode = 2016NatSR...620857K }}</ref>
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