Editing Semiconservative replication (section)

== Separation and recombination of double-stranded DNA ==
For semiconservative replication to occur, the DNA double-helix needs to be separated so the new template strand can be bound to the complementary base pairs. [[Topoisomerase]] is the enzyme that aids in the unzipping and recombination of the double-helix. Specifically, topoisomerase prevents the double-helix from supercoiling, or becoming too tightly wound. Three topoisomerase enzymes are involved in this process: [[Type I topoisomerase|Type IA Topoisomerase]], [[Type I topoisomerase|Type IB Topoisomerase]], and [[Type II topoisomerase|Type II Topoisomerase]].<ref>{{cite book |last=Brown |first=Terence A. | name-list-style = vanc | chapter-url= https://www.ncbi.nlm.nih.gov/books/NBK21113/ |chapter = Genome Replication | title = Genomes | edition = 2nd|date=2002|publisher=Wiley-Liss }}</ref> Type I Topoisomerase unwinds double stranded DNA while Type II Topoisomerase breaks the [[hydrogen bond]]s linking the complementary base pairs of DNA.<ref name = "Watson_2014">{{cite book | first1 = James D | last1 = Watson | first2 = Alexander | last2 = Gann | first3 = Tania A | last3 = Baker | first4 = Michael | last4 = Levine | first5 = Stephen P | last5 = Bell | first6 = Richard | last6 = Losick | name-list-style = vanc | date = 2014 |title=Molecular Biology of the Gene |isbn=978-0-321-76243-6 |edition=Seventh |location=Boston |oclc=824087979}}</ref>