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====Bisulfite sequencing==== Bisulfite sequencing is another way to evaluate DNA methylation. Cytosine will be changed to uracil from being treated with sodium bisulfite, whereas methylated cytosines will not be affected.<ref name="Hashimoto_2007" /><ref name="Li-Byarlay et al 2020">{{cite journal | vauthors = Li-Byarlay H, Boncristiani H, Howell G, Herman J, Clark L, Strand MK, Tarpy D, Rueppell O | title = Transcriptomic and Epigenomic Dynamics of Honey Bees in Response to Lethal Viral Infection | journal = Frontiers in Genetics | volume = 11 | pages = 566320 | date = 24 September 2020 | pmid = 33101388 | pmc = 7546774 | doi = 10.3389/fgene.2020.566320 | doi-access = free }}</ref><ref name="ReferenceC">{{cite journal | vauthors = Li-Byarlay H, Li Y, Stroud H, Feng S, Newman TC, Kaneda M, Hou KK, Worley KC, Elsik CG, Wickline SA, Jacobsen SE, Ma J, Robinson GE | title = RNA interference knockdown of DNA methyl-transferase 3 affects gene alternative splicing in the honey bee | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 110 | issue = 31 | pages = 12750β12755 | date = July 2013 | pmid = 23852726 | pmc = 3732956 | doi = 10.1073/pnas.1310735110 | doi-access = free | bibcode = 2013PNAS..11012750L }}</ref>
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