Editing Spider silk (section)

====Syringe and needle====
Feedstock is forced through a hollow needle using a syringe.<ref name="Lazaris 02">{{cite journal |author= Lazaris, A.|date= 2002 |title= Spider silk fibers spun from soluble recombinant silk produced in mammalian cells |journal= Science |volume= 295 |pages= 472–76 |doi=10.1126/science.1065780 |pmid=11799236|bibcode = 2002Sci...295..472L |issue= 5554 |first2= S |first3= Y |first4= JF |first5= F |first6= N |first7= EA |first8= JW |first9= CN  |last2= Arcidiacono, S |last3= Huang, Y |last4= Zhou, J. F. |last5= Duguay, F |last6= Chretien, N |last7= Welsh, E. A. |last8= Soares, J. W. |last9= Karatzas, C. N. |s2cid= 9260156 }}</ref><ref name="Seidel 00">{{cite journal |author= Seidel, A.|date= 2000 |title= Regenerated spider silk: Processing, properties, and structure |journal= Macromolecules |volume= 33 |pages= 775–80 |doi=10.1021/ma990893j |bibcode = 2000MaMol..33..775S |first2= Oskar |last3= Calve |first3= Sarah |last4= Adaska |first4= Jason |last5= Ji |first5= Gending |last6= Yang |first6= Zhitong |last7= Grubb |first7= David |last8= Zax |first8= David B. |last9= Jelinski |first9= Lynn W. |issue= 3 |last2= Liivak  }}</ref>

Although cheap and easy to produce, gland shape and conditions are loosely approximated. Fibres created using this method may need encouragement to solidify by removing water from the fibre with chemicals such as (environmentally undesirable) [[methanol]]<ref>{{cite journal |author= Arcidiacono, S.|date= 2002 |title= Aqueous processing and fiber spinning of recombinant spider silks |journal= Macromolecules |volume= 35 |pages= 1262–66 |doi=10.1021/ma011471o |bibcode = 2002MaMol..35.1262A |first2= Charlene M. |last3= Butler |first3= Michelle |last4= Welsh |first4= Elizabeth |last5= Soares |first5= Jason W. |last6= Allen |first6= Alfred |last7= Ziegler |first7= David |last8= Laue |first8= Thomas |last9= Chase |first9= Susan |issue= 4 |last2= Mello  }}</ref> or [[acetone]],<ref name="Seidel 00"/> and also may require later stretching of the fibre to achieve desirable properties.<ref name="Xia 10">{{cite journal |author= Xia, X. X.|date= 2010 |title= Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber |journal= Proceedings of the National Academy of Sciences of the United States of America |volume= 107 |pages= 14,059–63 |doi= 10.1073/pnas.1003366107|bibcode = 2010PNAS..10714059X |issue= 32 |display-authors=etal |pmid=20660779 |pmc=2922564|doi-access= free }}</ref><ref name="Lazaris 02"/>