Editing Western blot (section)

=== Minimum requirement specification for Western Blot ===
In order to ensure that the results of Western blots are reproducible, it is important to report the various parameters mentioned above, including specimen preparation, the concentration of protein used for loading, the percentage of gel and running condition, various transfer methods, attempting to block conditions, the concentration of antibodies, and identification and quantitative determination methods. Many of the articles that have been published don't cover all of these variables. Hence, it is crucial to describe different experimental circumstances or parameters in order to increase the repeatability and precision of WB. To increase WB repeatability, a minimum reporting criteria is thus required.<ref name=":3" /><ref>{{cite journal | vauthors = Gilda JE, Ghosh R, Cheah JX, West TM, Bodine SC, Gomes AV | title = Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS) | journal = PLOS ONE | volume = 10 | issue = 8 | pages = e0135392 | date = 2015-08-19 | pmid = 26287535 | pmc = 4545415 | doi = 10.1371/journal.pone.0135392 | doi-access = free | bibcode = 2015PLoSO..1035392G }}</ref>