Editing Nucleosome (section)

=== Disulfide crosslinked nucleosome core particles ===
A recent advance in the production of nucleosome core particles with enhanced stability involves site-specific [[disulfide]] crosslinks.<ref>{{cite journal | vauthors = Frouws TD, Barth PD, Richmond TJ | title = Site-Specific Disulfide Crosslinked Nucleosomes with Enhanced Stability | journal = Journal of Molecular Biology | volume = 430 | issue = 1 | pages = 45–57 | date = January 2018 | pmid = 29113904 | pmc = 5757783 | doi = 10.1016/j.jmb.2017.10.029 }}</ref> Two different crosslinks can be introduced into the nucleosome core particle. A first one crosslinks the two copies of [[Histone H2A|H2A]] via an introduced cysteine (N38C) resulting in [[histone octamer]] which is stable against H2A/H2B dimer loss during nucleosome reconstitution. A second crosslink can be introduced between the H3 N-terminal histone tail and the nucleosome DNA ends via an incorporated convertible nucleotide.<ref>{{cite book | vauthors = Ferentz AE, Verdine GL |chapter=The Convertible Nucleoside Approach: Structural Engineering of Nucleic Acids by Disulfide Cross-Linking |pages=14–40 |doi=10.1007/978-3-642-78666-2_2 | veditors = Eckstein F, Lilley DM |title=Nucleic Acids and Molecular Biology |volume=8 |year=1994 |isbn=978-3-642-78668-6 }}</ref> The DNA-histone octamer crosslink stabilizes the nucleosome core particle against DNA dissociation at very low particle concentrations and at elevated salt concentrations.