Editing Microsatellite (section)

===ISSR-PCR===
'''ISSR''' (for '''inter-simple sequence repeat''') is a general term for a genome region between microsatellite loci. The complementary sequences to two neighboring microsatellites are used as PCR primers<!-- Theor. Appl. Genet. 89, 998–1006. -->; the variable region between them gets amplified. The limited length of amplification cycles during PCR prevents excessive replication of overly long contiguous DNA sequences, so the result will be a mix of a variety of amplified DNA strands which are generally short but vary much in length.

Sequences amplified by ISSR-PCR can be used for DNA fingerprinting. Since an ISSR may be a conserved or nonconserved region, this technique is not useful for distinguishing individuals, but rather for [[phylogeography]] analyses<!-- Biota 3, 109–118. --> or maybe delimiting [[species]]<!-- Molecular Phylogenetics and Evolution 37 (2005) 389–401 -->; sequence diversity is lower than in SSR-PCR, but still higher than in actual gene sequences. In addition, microsatellite sequencing and ISSR sequencing are mutually assisting, as one produces primers for the other.