Editing Western blot (section)

=== Identification of protein localization across cells ===
For medication development, the identification of therapeutic targets, and biological research, it is essential to comprehend where proteins are located within a cell.<ref name=":3" /><ref>{{cite journal | vauthors = Lee YH, Tan HT, Chung MC | title = Subcellular fractionation methods and strategies for proteomics | journal = Proteomics | volume = 10 | issue = 22 | pages = 3935–3956 | date = November 2010 | pmid = 21080488 | doi = 10.1002/pmic.201000289 | s2cid = 29256675 }}</ref> The subcellular locations of proteins inside the cell and their functions are closely related. The relationship between protein function and localization suggests that when proteins move, their functions may change or acquire new characteristics. A protein's subcellular placement can be determined using a variety of methods. Numerous efficient and reliable computational tools and strategies have been created and used to identify protein subcellular localization.<ref>{{cite book | vauthors = Barberis E, Marengo E, Manfredi M | title = Proteomics Data Analysis | chapter = Protein Subcellular Localization Prediction | series = Methods in Molecular Biology | volume = 2361 | pages = 197–212 | date = 2021 | pmid = 34236663 | doi = 10.1007/978-1-0716-1641-3_12 | publisher = Springer US | isbn = 978-1-0716-1640-6 | s2cid = 235768807 | place = New York, NY | veditors = Cecconi D }}</ref> With the aid of subcellular fractionation methods, WB continues to be an important fundamental method for the investigation and comprehension of protein localization.<ref name=":3" />