Editing Western blot (section)

==== Fluorescent detection ====
[[File:Anti-lipoic acid immunoblot.png|thumb|Western blot using an anti-[[lipoic acid]] primary antibody and an [[infrared|IR]]-dye labelled secondary antibody in ''[[Leishmania]]'' major extracts]]
The [[Fluorescent tag|fluorescently labeled]] probe is excited by light and the emission of the excitation is then detected by a photosensor such as a CCD camera equipped with appropriate emission filters which captures a digital image of the western blot and allows further data analysis such as molecular weight analysis and a quantitative western blot analysis. Fluorescence is considered to be one of the best methods for quantification but is less sensitive than chemiluminescence.<ref>{{cite book | vauthors = Mathews ST, Plaisance EP, Kim T | chapter = Imaging Systems for Westerns: Chemiluminescence vs. Infrared Detection | title = Protein Blotting and Detection | series = Methods in Molecular Biology | volume = 536 | pages = 499–513 | year = 2009 | pmid = 19378087 | doi = 10.1007/978-1-59745-542-8_51 | publisher = Humana Press | isbn = 978-1-934115-73-2 }}</ref>