Editing Biotin (section)

==Interference with medical laboratory results==
When people are ingesting high levels of biotin in [[dietary supplement]]s, a consequence can be clinically significant interference with [[diagnosis|diagnostic]] blood tests that use biotin-streptavidin technology. This methodology is commonly used to measure levels of hormones such as [[thyroid hormones]], and other analytes such as 25-hydroxyvitamin D. Biotin interference can produce both falsely normal and falsely abnormal results.<ref name=ods/><ref name=FDA2017>{{cite web|title=The FDA Warns that Biotin May Interfere with Lab Tests: FDA Safety Communication|date=28 November 2017|url=https://www.fda.gov/MedicalDevices/Safety/AlertsandNotices/ucm586505.htm|publisher=US Food and Drug Administration|access-date=5 January 2021|archive-date=April 24, 2019|archive-url=https://web.archive.org/web/20190424073101/https://www.fda.gov/MedicalDevices/Safety/AlertsandNotices/ucm586505.htm|url-status=live}}</ref> In the US, biotin as a non-prescription dietary supplement is sold in amounts of 1 to 10&nbsp;mg per serving, with claims for supporting hair and nail health, and as 300&nbsp;mg per day as a possibly effective treatment for multiple sclerosis<ref name=Tryfonow2019/><ref name=Sedel2016/> (see [[Biotin#Research|§ Research]]). Overconsumption of 5&nbsp;mg/day or higher causes elevated concentration in plasma that interferes with biotin-streptavidin immunoassays in an unpredictable manner.<ref name=Luong2019>{{cite journal |vauthors=Luong JH, Vashist SK |title=Chemistry of Biotin-Streptavidin and the Growing Concern of an Emerging Biotin Interference in Clinical Immunoassays |journal=ACS Omega |volume=5 |issue=1 |pages=10–18 |date=January 2020 |pmid=31956746 |pmc=6963918 |doi=10.1021/acsomega.9b03013 |url=}}</ref><ref name=Bowen2019>{{cite journal |vauthors=Bowen R, Benavides R, Colón-Franco JM, Katzman BM, Muthukumar A, Sadrzadeh H, Straseski J, Klause U, Tran N |title=Best practices in mitigating the risk of biotin interference with laboratory testing |journal=Clin Biochem |volume=74 |issue= |pages=1–11 |date=December 2019 |pmid=31473202 |doi=10.1016/j.clinbiochem.2019.08.012 |url=|doi-access=free }}</ref> Healthcare professionals are advised to instruct patients to stop taking biotin supplements for 48 h or even up to weeks before the test, depending on the specific test, dose, and frequency of biotin uptake.<ref name=Luong2019/> Guidance for laboratory staff is proposed to detect and manage biotin interference.<ref name=Bowen2019/>