Editing Molecular biology (section)

====Northern blotting====
{{main|Northern blot}}
[[File:Northern blot diagram.png|thumb|Northern blot diagram]]
The northern blot is used to study the presence of specific RNA molecules as relative comparison among a set of different samples of RNA. It is essentially a combination of [[denaturing gel|denaturing RNA gel electrophoresis]], and a [[blot (biology)|blot]]. In this process RNA is separated based on size and is then transferred to a membrane that is then probed with a labeled [[complementarity (molecular biology)|complement]] of a sequence of interest. The results may be visualized through a variety of ways depending on the label used; however, most result in the revelation of bands representing the sizes of the RNA detected in sample. The intensity of these bands is related to the amount of the target RNA in the samples analyzed. The procedure is commonly used to study when and how much gene expression is occurring by measuring how much of that RNA is present in different samples, assuming that no post-transcriptional regulation occurs and that the levels of mRNA reflect proportional levels of the corresponding protein being produced. It is one of the most basic tools for determining at what time, and under what conditions, certain genes are expressed in living tissues.<ref>{{cite book |doi=10.1007/978-1-59745-248-9_7 |chapter=Northern Blotting Analysis |title=RNA |series=Methods in Molecular Biology |date=2011 |last1=Josefsen |first1=Knud |last2=Nielsen |first2=Henrik |volume=703 |pages=87–105 |pmid=21125485 |isbn=978-1-58829-913-0 }}</ref><ref>{{cite book | vauthors = He SL, Green R | title = Laboratory Methods in Enzymology: RNA | chapter = Northern blotting | volume = 530 | pages = 75–87 | date = 1 January 2013 | pmid = 24034315 | pmc = 4287216 | doi = 10.1016/B978-0-12-420037-1.00003-8 | isbn = 978-0-12-420037-1 }}</ref>