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==== Single plane illumination microscopy and light sheet fluorescence microscopy ==== {{Main|Light sheet fluorescence microscopy}} Using a plane of light formed by focusing light through a cylindrical lens at a narrow angle or by scanning a line of light in a plane perpendicular to the axis of objective, high resolution optical sections can be taken.<ref>{{Cite journal| doi = 10.1016/S0378-5955(02)00493-8| pmid = 12204356| issn = 0378-5955| volume = 71| pages = 119β128| last = Voie| first = A.H.| title = Imaging the intact guinea pig tympanic bulla by orthogonal-plane fluorescence optical sectioning microscopy| journal = Hearing Research| year = 1993| issue = 1β2| s2cid = 12775304}}</ref><ref>{{Cite journal| doi = 10.1063/1.2428277| issn = 0034-6748| volume = 78| last = Greger| first = K.| author2 = J. Swoger| author3 = E. H. K. Stelzer| title = Basic building units and properties of a fluorescence single plane illumination microscope| journal = Review of Scientific Instruments| year = 2007| issue = 2| pages = 023705β023705β7| pmid = 17578115| bibcode = 2007RScI...78b3705G| doi-access = free}}</ref><ref>{{Cite journal| doi = 10.1155/2012/206238| pmid = 22567307| pmc = 3335623| issn = 2090-2743| volume = 2012| page = 206238| last = Buytaert| first = J.A.N. |author2=E. Descamps |author3=D. Adriaens |author4=J.J.J. Dirckx| title = The OPFOS Microscopy Family: High-Resolution Optical Sectioning of Biomedical Specimens| journal = Anatomy Research International| year = 2012| arxiv = 1106.3162| doi-access = free}}</ref> Single plane illumination, or light sheet illumination, is also accomplished using [[Beam expander#Extra-cavity beam shaping|beam shaping techniques incorporating multiple-prism beam expanders]].<ref>F. J. Duarte, in ''High Power Dye Lasers'' (Springer-Verlag, Berlin,1991) Chapter 2.</ref><ref>[[F. J. Duarte|Duarte FJ]] (1993), Electro-optical interferometric microdensitometer system, [http://www.patentgenius.com/patent/5255069.html ''US Patent'' 5255069] {{Webarchive|url=https://web.archive.org/web/20171013042159/http://www.patentgenius.com/patent/5255069.html |date=2017-10-13 }}.</ref> The images are captured by CCDs. These variants allow very fast and high signal to noise ratio image capture.
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