Editing Azadirachta indica (section)

==Biotechnology==
The biopesticide produced by extraction from the tree seeds contains [[limonoid]] [[triterpene]]s.<ref name=drugs/> Currently, the extraction process has disadvantages such as contamination with fungi and heterogeneity in the content of limonoids due to genetic, climatic, and geographical variations.<ref>{{Cite journal|last1=Sidhu|first1=O. P.|last2=Kumar|first2=Vishal|last3=Behl|first3=Hari M.|date=15 January 2003|title=Variability in Neem (''Azadirachta indica'') with Respect to Azadirachtin Content|journal=Journal of Agricultural and Food Chemistry|language=en|volume=51|issue=4|pages=910–915|doi=10.1021/jf025994m|pmid=12568548|bibcode=2003JAFC...51..910S }}</ref><ref>{{Cite journal|last1=Prakash|first1=Gunjan|last2=Bhojwani|first2=Sant S.|last3=Srivastava|first3=Ashok K.|s2cid=85845199|date=1 August 2002|title=Production of azadirachtin from plant tissue culture: State of the art and future prospects|journal=Biotechnology and Bioprocess Engineering|language=en|volume=7|issue=4|pages=185–193|doi=10.1007/BF02932968|issn=1226-8372}}</ref> To overcome these problems, production of limonoids from plant [[cell suspension]] and hairy root cultures in bioreactors has been studied,<ref>{{Cite journal|last1=Srivastava|first1=Smita|last2=Srivastava|first2=Ashok K.|s2cid=36781838|date=17 August 2013|title=Production of the Biopesticide Azadirachtin by Hairy Root Cultivation of Azadirachta indica in Liquid-Phase Bioreactors|journal=Applied Biochemistry and Biotechnology|language=en|volume=171|issue=6|pages=1351–1361|doi=10.1007/s12010-013-0432-7|pmid=23955295|issn=0273-2289}}</ref><ref>{{Cite journal|last1=Prakash|first1=Gunjan|last2=Srivastava|first2=Ashok K.|s2cid=35506559|date=5 April 2008|title=Production of Biopesticides in an in Situ Cell Retention Bioreactor|journal=Applied Biochemistry and Biotechnology|language=en|volume=151|issue=2–3|pages=307–318|doi=10.1007/s12010-008-8191-6|pmid=18392561|issn=0273-2289}}</ref> including the development of a two-stage bioreactor process that enhances growth and production of limonoids with cell suspension cultures of ''A. indica''.<ref>{{Cite journal|last1=Vásquez-Rivera|first1=Andrés|last2=Chicaiza-Finley|first2=Diego|last3=Hoyos|first3=Rodrigo A.|last4=Orozco-Sánchez|first4=Fernando|s2cid=207357717|date=1 September 2015|title=Production of Limonoids with Insect Antifeedant Activity in a Two-Stage Bioreactor Process with Cell Suspension Culture of Azadirachta indica|journal=Applied Biochemistry and Biotechnology|volume=177|issue=2|pages=334–345|doi=10.1007/s12010-015-1745-5|issn=1559-0291|pmid=26234433}}</ref>