Editing Green fluorescent protein (section)

=== Macro-photography ===
Macro-scale biological processes, such as the spread of virus infections, can be followed using GFP labeling.<ref>{{cite journal | vauthors = Rodman MK, Yadav NS, Artus NN | title = Progression of geminivirus-induced transgene silencing is associated with transgene methylation | journal = The New Phytologist | volume = 155 | issue = 3 | pages = 461–468 | date = September 2002 | pmid = 33873315 | doi = 10.1046/j.1469-8137.2002.00467.x | bibcode = 2002NewPh.155..461R }}</ref>  In the past, mutagenic ultra violet light (UV) has been used to illuminate living organisms  (e.g., see<ref>{{cite journal | vauthors = Zhu YJ, Agbayani R, Moore PH | title = Green fluorescent protein as a visual selection marker for papaya (Carica papaya L.) transformation | journal = Plant Cell Reports | volume = 22 | issue = 9 | pages = 660–7 | date = Apr 2004 | pmid = 14749892 | doi = 10.1007/s00299-004-0755-5 | s2cid = 23198182 }}</ref>) to detect and photograph the GFP expression. Recently, a technique using non-mutagenic LED lights<ref name="pmid10406127">{{cite journal | vauthors = Niwa Y, Hirano T, Yoshimoto K, Shimizu M, Kobayashi H | s2cid = 292648 | title = Non-invasive quantitative detection and applications of non-toxic, S65T-type green fluorescent protein in living plants | journal = The Plant Journal | volume = 18 | issue = 4 | pages = 455–63 | year = 1999 | pmid = 10406127 | doi = 10.1046/j.1365-313X.1999.00464.x | doi-access = free }}</ref> have been developed for macro-photography.<ref>{{cite journal | vauthors = Baker SS, Vidican CB, Cameron DS, Greib HG, Jarocki CC, Setaputri AW, Spicuzza CH, Burr AA, Waqas MA, Tolbert DA | title = An epifluorescent attachment improves whole-plant digital photography of Arabidopsis thaliana expressing red-shifted green fluorescent protein | journal = AoB Plants | volume = 2012 | pages = pls003 | date = 2012-01-01 | pmid = 22479674 | pmc = 3296078 | doi = 10.1093/aobpla/pls003 }}</ref>  The technique uses an epifluorescence camera attachment<ref>{{cite web | url = http://planted.botany.org/index.php?P=FullRecord&ID=570 | title = PlantEdDL - Using SRL digital cameras in quantitative investigations of plants expressing green fluorescent protein (GFP) | website = planted.botany.org | access-date = 2016-03-23 }}</ref> based on the same principle used in the construction of [[Fluorescence microscope|epifluorescence microscopes]].