Jump to content
Main menu
Main menu
move to sidebar
hide
Navigation
Main page
Recent changes
Random page
Help about MediaWiki
Special pages
Niidae Wiki
Search
Search
Appearance
Create account
Log in
Personal tools
Create account
Log in
Pages for logged out editors
learn more
Contributions
Talk
Editing
Restriction enzyme
(section)
Page
Discussion
English
Read
Edit
View history
Tools
Tools
move to sidebar
hide
Actions
Read
Edit
View history
General
What links here
Related changes
Page information
Appearance
move to sidebar
hide
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
== Examples == {{See also|List of restriction enzyme cutting sites}} Examples of restriction enzymes include:<ref name="pmid6243774">{{cite journal | vauthors = Roberts RJ | title = Restriction and modification enzymes and their recognition sequences | journal = Nucleic Acids Research | volume = 8 | issue = 1 | pages = r63βr80 | date = January 1980 | pmid = 6243774 | pmc = 327257 | doi = 10.1093/nar/8.1.197-d }}</ref> {| class="wikitable sortable" style="border:1px solid #aaa;" |- ! Enzyme !! Source !! Recognition Sequence !! Cut |- |[[EcoRI]]||''[[Escherichia coli]]'' | 5'GAATTC 3'CTTAAG | 5'---G AATTC---3' 3'---CTTAA G---5' |- |[[EcoRII]]||''[[Escherichia coli]]'' | 5'CCWGG 3'GGWCC | 5'--- CCWGG---3' 3'---GGWCC ---5' |- |[[BamHI]]||''[[Bacillus amyloliquefaciens]]'' | 5'GGATCC 3'CCTAGG | 5'---G GATCC---3' 3'---CCTAG G---5' |- |[[HindIII]]||''[[Haemophilus influenzae]]'' | 5'AAGCTT 3'TTCGAA | 5'---A AGCTT---3' 3'---TTCGA A---5' |- |[[TaqI]]||''[[Thermus aquaticus]]'' | 5'TCGA 3'AGCT | 5'---T CGA---3' 3'---AGC T---5' |- |[[NotI]]||''[[Nocardia otitidis]]'' | 5'GCGGCCGC 3'CGCCGGCG | 5'---GC GGCCGC---3' 3'---CGCCGG CG---5' |- |[[HinFI]]||''[[Haemophilus influenzae]]'' | 5'GANTC 3'CTNAG | 5'---G ANTC---3' 3'---CTNA G---5' |- |[[Sau3AI]]||''[[Staphylococcus aureus]]'' | 5'GATC 3'CTAG | 5'--- GATC---3' 3'---CTAG ---5' |- |[[PvuII|PvuII*]]||''[[Proteus vulgaris]]'' | 5'CAGCTG 3'GTCGAC | 5'---CAG CTG---3' 3'---GTC GAC---5' |- |[[SmaI|SmaI*]]||''[[Serratia marcescens]]'' | 5'CCCGGG 3'GGGCCC | 5'---CCC GGG---3' 3'---GGG CCC---5' |- |[[HaeIII|HaeIII*]]||''[[Haemophilus influenzae biogroup aegyptius|Haemophilus aegyptius]]'' | 5'GGCC 3'CCGG | 5'---GG CC---3' 3'---CC GG---5' |- |[[HgaI]]<ref name="pmid2835753">{{cite journal | vauthors = Roberts RJ | title = Restriction enzymes and their isoschizomers | journal = Nucleic Acids Research | volume = 16 Suppl | issue = Suppl | pages = r271-313 | year = 1988 | pmid = 2835753 | pmc = 340913 | doi = 10.1093/nar/16.suppl.r271 }}</ref>||''[[Haemophilus gallinarum]]'' | 5'GACGC 3'CTGCG | 5'---NN NN---3' 3'---NN NN---5' |- |[[AluI|AluI*]]||''[[Arthrobacter luteus]]'' | 5'AGCT 3'TCGA | 5'---AG CT---3' 3'---TC GA---5' |- |[[EcoRV|EcoRV*]]||''[[Escherichia coli]]'' | 5'GATATC 3'CTATAG | 5'---GAT ATC---3' 3'---CTA TAG---5' |- |[[EcoP15I]]||''[[Escherichia coli]]'' | 5'CAGCAGN<sub>25</sub>NN 3'GTCGTCN<sub>25</sub>NN | 5'---CAGCAGN<sub>25</sub> NN---3' 3'---GTCGTCN<sub>25</sub>NN ---5' |- |[[KpnI]]<ref name="isbn0-7167-4366-3">{{cite book | vauthors = Krieger M, Scott MP, Matsudaira PT, Lodish HF, Darnell JE, Zipursky L, Kaiser C, Berk A | title = Molecular Cell Biology | edition = 5th | publisher = W.H. Freeman and Company | location = New York | year = 2004 | isbn = 0-7167-4366-3 | url = https://archive.org/details/molecularcellbio00harv }}</ref>||''[[Klebsiella pneumoniae]]'' | 5'GGTACC 3'CCATGG | 5'---GGTAC C---3' 3'---C CATGG---5' |- |[[PstI]]<ref name="isbn0-7167-4366-3"/>||''[[Providencia stuartii]]'' | 5'CTGCAG 3'GACGTC | 5'---CTGCA G---3' 3'---G ACGTC---5' |- |[[SacI]]<ref name="isbn0-7167-4366-3"/>||''[[Streptomyces achromogenes]]'' | 5'GAGCTC 3'CTCGAG | 5'---GAGCT C---3' 3'---C TCGAG---5' |- |[[SalI]]<ref name="isbn0-7167-4366-3"/>||''[[Streptomyces albus]]'' | 5'GTCGAC 3'CAGCTG | 5'---G TCGAC---3' 3'---CAGCT G---5' |- |[[ScaI]]*<ref name="isbn0-7167-4366-3"/>||''[[Streptomyces caespitosus]]'' | 5'AGTACT 3'TCATGA | 5'---AGT ACT---3' 3'---TCA TGA---5' |- |[[SpeI]]||''[[Sphaerotilus natans]]'' | 5'ACTAGT 3'TGATCA | 5'---A CTAGT---3' 3'---TGATC A---5' |- |[[SphI]]<ref name="isbn0-7167-4366-3"/>||''[[Streptomyces phaeochromogenes]]'' | 5'GCATGC 3'CGTACG | 5'---GCATG C---3' 3'---C GTACG---5' |- |[[StuI]]*<ref name="Sigma_R8013">{{cite web | url = http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/R8013 | title = Stu I from Streptomyces tubercidicus |publisher = Sigma-Aldrich |access-date=2008-06-07}}</ref><ref name="pmid6260571">{{cite journal | vauthors = Shimotsu H, Takahashi H, Saito H | title = A new site-specific endonuclease StuI from Streptomyces tubercidicus | journal = Gene | volume = 11 | issue = 3β4 | pages = 219β25 | date = November 1980 | pmid = 6260571 | doi = 10.1016/0378-1119(80)90062-1 }}</ref>||''[[Streptomyces tubercidicus]]'' | 5'AGGCCT 3'TCCGGA | 5'---AGG CCT---3' 3'---TCC GGA---5' |- |[[XbaI]]<ref name="isbn0-7167-4366-3"/>||''[[Xanthomonas badrii]]'' | 5'TCTAGA 3'AGATCT | 5'---T CTAGA---3' 3'---AGATC T---5' |} Key:<br/> <nowiki>*</nowiki> = blunt ends<br/> N = C or G or T or A<br/> W = A or T
Summary:
Please note that all contributions to Niidae Wiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
Encyclopedia:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Search
Search
Editing
Restriction enzyme
(section)
Add topic