Editing Catalase (section)

== Methods for determining catalase activity ==
In 1870, Schoenn discovered a formation of yellow color from the interaction of hydrogen peroxide with molybdate;<ref>{{Cite journal| vauthors = Isaacs ML |date=1922|title=A colorimetric determination of hydrogen peroxide|url=https://pubs.acs.org/doi/pdf/10.1021/ja01429a006|journal=Journal of the American Chemical Society|language=|volume=44|issue=8|pages=1662–1663|doi=10.1021/ja01429a006|bibcode=1922JAChS..44.1662I |s2cid= |issn=}}</ref> then, from the middle of the 20th century, this reaction began to be used for colorimetric determination of unreacted hydrogen peroxide in the catalase activity assay.<ref>{{Cite journal| vauthors = Peizer LR, Widelock D |date=1955|title=A colorimetric test for measuring catalase activity of cultures of M. tuberculosis|url=https://www.atsjournals.org/doi/abs/10.1164/artpd.1955.71.2.305 |journal= American Review of Tuberculosis|language=|volume=71|issue=2|pages=305–313|doi=10.1164/artpd.1955.71.2.305|doi-broken-date=1 November 2024 |pmid=14350192 |s2cid= |issn=}}</ref> The reaction became widely used after publications by Korolyuk et al. (1988)<ref>{{cite journal | vauthors = Koroliuk MA, Ivanova LI, Maĭorova IG, Tokarev VE | title = [A method of determining catalase activity] | journal = Laboratornoe Delo | issue = 1 | pages = 16–19 | date = 1988 | pmid = 2451064 | url = https://pubmed.ncbi.nlm.nih.gov/2451064/ }}</ref> and Goth (1991).<ref name="pmid2029780">{{cite journal | vauthors = Góth L | title = A simple method for determination of serum catalase activity and revision of reference range | journal = Clinica Chimica Acta; International Journal of Clinical Chemistry | volume = 196 | issue = 2–3 | pages = 143–151 | date = February 1991 | pmid = 2029780 | doi = 10.1016/0009-8981(91)90067-m }}</ref> The first paper describes serum catalase assay with no buffer in the reaction medium; the latter describes the procedure based on phosphate buffer as a reaction medium. Since phosphate ion reacts with ammonium molybdate,<ref name="pmid2029780" /> the use of MOPS buffer as a reaction medium is more appropriate.<ref>{{cite journal | vauthors = Razygraev AV | title = Catalase enzymatic activity in adult mosquitoes (Diptera: Culicidae): taxonomic distribution of the continuous trait suggests its relevance for phylogeny research |url=https://www.mapress.com/zt/article/view/zootaxa.5339.2.3 | journal = Zootaxa | volume = 5339 | issue = 2 | pages = 159–176 | date = 2023 | pmid = 38221060| doi = 10.11646/zootaxa.5339.2.3 | s2cid = 261383164 }}</ref>

Direct UV measurement of the decrease in the concentration of hydrogen peroxide is also widely used after the publications by Beers & Sizer<ref>{{cite journal | vauthors = Beers RF, Sizer IW | title = A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase | journal = The Journal of Biological Chemistry | volume = 195 | issue = 1 | pages = 133–140 | date = March 1952 | doi = 10.1016/S0021-9258(19)50881-X | pmid = 14938361 | doi-access = free }}</ref> and Aebi.<ref>{{cite book | vauthors = Aebi H | chapter = Catalase in vitro |title=Oxygen Radicals in Biological Systems | series = Methods in Enzymology | volume = 105 | pages = 121–126 | date = January 1984 | pmid = 6727660 | doi = 10.1016/s0076-6879(84)05016-3 | publisher = Academic Press | isbn = 9780121820053 }}</ref>