Editing Glycoprotein (section)

=== Glycosylation ===
The formation of the link between the glycan and the protein is key element of the synthesis of glycoproteins.<ref name="Gamblin_2009" /> The most common method of glycosylation of N-linked glycoproteins is through the reaction between a protected glycan and a protected Asparagine.<ref name="Gamblin_2009" /> Similarly, an O-linked glycoprotein can be formed through the addition of a [[glycosyl]] donor with a protected [[Serine]] or [[Threonine]].<ref name="Gamblin_2009" /> These two methods are examples of natural linkage.<ref name="Gamblin_2009" /> However, there are also methods of unnatural linkages.<ref name="Gamblin_2009" /> Some methods include ligation and a reaction between a serine-derived sulfamidate and thiohexoses in water.<ref name="Gamblin_2009" /> Once this linkage is complete, the amino acid sequence can be expanded upon using solid-phase peptide synthesis.<ref name="Gamblin_2009" />