Editing Glycoprotein (section)

=== Recombination ===
[[File:Variety of glycans.svg|thumb|337x337px|Depiction of differences in glycans amongst different animals.]]
There are several techniques for the assembly of glycoproteins. One technique utilizes [[Recombinant DNA|recombination]].<ref name="Picanco_e_Castro_2018" /> The first consideration for this method is the choice of host, as there are many different factors that can influence the success of glycoprotein recombination such as cost, the host environment, the efficacy of the process, and other considerations.<ref name="Picanco_e_Castro_2018" /> Some examples of host cells include E. coli, yeast, plant cells, insect cells, and mammalian cells.<ref name="Picanco_e_Castro_2018" /> Of these options, mammalian cells are the most common because their use does not face the same challenges that other host cells do such as different glycan structures, shorter half life, and potential unwanted immune responses in humans.<ref name="Picanco_e_Castro_2018" /> Of mammalian cells, the most common cell line used for recombinant glycoprotein production is the [[Chinese hamster ovary cell|Chinese hamster ovary]] line.<ref name="Picanco_e_Castro_2018" /> However, as technologies develop, the most promising cell lines for recombinant glycoprotein production are human cell lines.<ref name="Picanco_e_Castro_2018" />