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===General=== [[File:Vulva with vaginal fluid (ejaculation - squirting).png|thumb|Vaginal fluids after ejaculation]] There have been few studies on female ejaculation.<ref name="Estupinyà"/> Much of the problem in arriving at a consensus relates to a failure to adopt generally agreed-on definitions<ref name="Taverner"/><ref name="schubach02">{{cite journal |author=Schubach G |title=The G-spot is the female prostate |journal=Am. J. Obstet. Gynecol. |volume=186 |issue=4 |pages=850; author reply 850 |date=April 2002 |pmid=11967519 |doi= 10.1067/mob.2002.121628|doi-access=free }}</ref> or research methodology.<ref name="Taverner"/> Research has used highly selected individuals, case studies, or very small numbers of subjects, making generalization difficult. For instance, much of the research into the nature of the fluid focuses on determining whether it is or contains urine.<ref name="Estupinyà"/><ref name="Taverner"/> Rodriguez et al. stated that "many individuals refer to any fluid expressed from the vagina or urethra during coitus as female ejaculate, which leads to significant confusion in the literature."<ref name="rodriguez"/> There are also problems involved in the collection of specimens and issues of contamination. Since the area of interest is the para-urethral glands, it is impossible to completely separate the secretions from urine, especially considering that there may be [[retrograde ejaculation]] into the urethra towards the bladder. Research has attempted to use chemicals that are excreted in the urine so that any urinary contamination can be detected. Further methodological issues include the fact that the composition of the fluid appears to vary with the menstrual cycle,<ref>{{cite journal |author=Zaviacic M |title=The fluid of female urethral expulsions analysed by histochemical electron-microscopic and other methods |journal=Histochem. J. |volume=16 |issue=4 |pages=445–7 |date=April 1984 |pmid=6538874 |doi= 10.1007/BF01002874|author2=Jakubovský J |author3=Polák S |display-authors=3 |last4=Zaviačičová |first4=A. |last5=Holomáň |first5=I. K. |last6=Blazekova |first6=J. |last7=Gregor |first7=P.|s2cid=27878970 }}</ref> and that the biochemical profile of the para-urethral tissues varies with age.<ref>{{cite journal |vauthors=Zaviacic M, Porubský J, Vierik J, Holomán IK |title=[Enzymes of the female prostate during the fertile age and after menopause. Comparative histochemical study] |language=sk |journal=Cesk Gynekol |volume=54 |issue=10 |pages=755–60 |date=December 1989 |pmid=2630042 }}</ref> Other issues relate to the sensitivity and specificity of the markers chosen. The key questions are the source of the fluid produced, and its composition.<ref name="bohlen"/> Whether the fluid is secreted by the Skene's gland through and around the urethra has also been a topic of discussion; while the exact source and nature of the fluid remain controversial among medical professionals, and are related to doubts over the existence of the [[G-spot]],<ref name="Balon, Segraves"/><ref name="Greenberg"/><ref name="rodriguez"/> there is substantial evidence that the Skene's gland is the source of female ejaculation.<ref name=pastor/><ref name="rodriguez"/> The function of female ejaculation, however, remains unclear.<ref name="rodriguez"/>
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