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=== X-ray microanalysis === {{Main|Energy-dispersive X-ray spectroscopy}} [[File:EDS_-_Rimicaris_exoculata.png|thumb|class=skin-invert-image|EDS spectrum of the mineral crust of the vent shrimp ''[[Rimicaris exoculata]]''<ref>{{cite journal |author=Corbari, L |display-authors=etal |year=2008 |title=Iron oxide deposits associated with the ectosymbiotic bacteria in the hydrothermal vent shrimp Rimicaris exoculata |journal=Biogeosciences |volume=5 |issue=5 |pages=1295β1310 |bibcode=2008BGeo....5.1295C |doi=10.5194/bg-5-1295-2008 |doi-access=free}}</ref> Most of these peaks are [[K-alpha]] and [[K-beta]] lines. One peak is from the L shell of iron.]] X-ray microanalysis is a method of obtaining local chemical information within electron microscopes or all types, although it is most commonly used in scanning instruments. When high energy electrons interact with atoms they can knock out electrons, particularly those in the [[inner shell]]s and [[core electrons]]. These are then filled by [[valence electron]], and the energy difference between the valence and core states can be converted into an [[x-ray]] which is detected by a spectrometer. The energies of these x-rays is somewhat specific to the atomic species, so local chemistry can be probed.<ref name=":1">{{Cite book |last=Goldstein |first=Joseph |url=https://books.google.com/books?id=ruF9DQxCDLQC |title=Scanning Electron Microscopy and X-Ray Microanalysis: Third Edition |date=2003-01-31 |publisher=Springer US |isbn=978-0-306-47292-3 |language=en}}</ref>
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