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==== ''Enterococcus faecalis'' ==== ''[[Enterococcus faecalis]]'' is an opportunistic, gram-positive bacteria that forms biofilm in glass. This process is also known as forming a biofilm in vitro. The presence of (Esp), a certain cell surface protein, aids the formation of a biofilm by ''E. faecalis''.<ref>{{cite journal | pmc=365672 | date=2004 | last1=Kristich | first1=C. J. | last2=Li | first2=Y. H. | last3=Cvitkovitch | first3=D. G. | last4=Dunny | first4=G. M. | title=Esp-Independent Biofilm Formation by Enterococcus faecalis | journal=Journal of Bacteriology | volume=186 | issue=1 | pages=154–163 | doi=10.1128/JB.186.1.154-163.2004 | pmid=14679235 }}</ref> The ability of ''E. faecalis'' to form [[biofilm]]s contributes to its capacity to survive in extreme environments, and facilitates its involvement in persistent bacterial infection, particularly in the case of multi-drug resistant strains.<ref name = Schiopu2023>{{cite journal |vauthors=Șchiopu P, Toc DA, Colosi IA, Costache C, Ruospo G, Berar G, Gălbău ȘG, Ghilea AC, Botan A, Pană AG, Neculicioiu VS, Todea DA |title=An Overview of the Factors Involved in Biofilm Production by the Enterococcus Genus |journal=Int J Mol Sci |volume=24 |issue=14 |date=July 2023 |page=11577 |pmid=37511337 |pmc=10380289 |doi=10.3390/ijms241411577 |doi-access=free |url=}}</ref> Biofilm formation in ''E. faecalis'' is associated with [[DNA]] release, and such release has emerged as a fundamental aspect of biofilm formation.<ref name = Schiopu2023/> Conjugative [[plasmid]] DNA transfer in ''E. faecalis'' is enhanced by the release of peptide [[sex pheromone]]s.<ref>{{cite journal |vauthors=Hirt H, Greenwood-Quaintance KE, Karau MJ, Till LM, Kashyap PC, Patel R, Dunny GM |title=Enterococcus faecalis Sex Pheromone cCF10 Enhances Conjugative Plasmid Transfer In Vivo |journal=mBio |volume=9 |issue=1 |pages= |date=February 2018 |pmid=29440568 |pmc=5821081 |doi=10.1128/mBio.00037-18 |url=}}</ref>
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