Editing Plasmid (section)

===Protein Production===
{{main|Expression vector}}
[[File:Human insulin 100IU-ml vial white background.jpg|thumb|Insulin]]
Another major use of plasmids is to make large amounts of proteins. In this case, researchers grow bacteria containing a plasmid harboring the gene of interest. Just as the bacterium produces proteins to confer its antibiotic resistance, it can also be induced to produce large amounts of proteins from the inserted gene. This is a cheap and easy way of mass-producing the protein, for example, utilizing the rapid reproduction of E.coli with a plasmid containing the [[insulin]] gene leads to a large production of insulin.<ref>{{Cite journal |last1=Goeddel |first1=D V |last2=Kleid |first2=D G |last3=Bolivar |first3=F |last4=Heyneker |first4=H L |last5=Yansura |first5=D G |last6=Crea |first6=R |last7=Hirose |first7=T |last8=Kraszewski |first8=A |last9=Itakura |first9=K |last10=Riggs |first10=A D |date=January 1979 |title=Expression in Escherichia coli of chemically synthesized genes for human insulin. |journal=Proceedings of the National Academy of Sciences |volume=76 |issue=1 |pages=106–110 |doi=10.1073/pnas.76.1.106 |doi-access=free |pmc=382885 |pmid=85300 |bibcode=1979PNAS...76..106G }}</ref><ref>{{cite journal |last1=Govender |first1=Kamini |last2=Naicker |first2=Tricia |last3=Lin |first3=Johnson |last4=Baijnath |first4=Sooraj |last5=Chuturgoon |first5=Anil Amichund |last6=Abdul |first6=Naeem Sheik |last7=Docrat |first7=Taskeen |last8=Kruger |first8=Hendrik Gerhardus |last9=Govender |first9=Thavendran |title=A novel and more efficient biosynthesis approach for human insulin production in Escherichia coli (E. coli) |journal=AMB Express |date=December 2020 |volume=10 |issue=1 |page=43 |doi=10.1186/s13568-020-00969-w |doi-access=free |pmc=7062966 |pmid=32152803 }}</ref><ref>{{Cite journal |last1=Zieliński |first1=Marcin |last2=Romanik-Chruścielewska |first2=Agnieszka |last3=Mikiewicz |first3=Diana |last4=Łukasiewicz |first4=Natalia |last5=Sokołowska |first5=Iwona |last6=Antosik |first6=Jarosław |last7=Sobolewska-Ruta |first7=Agnieszka |last8=Bierczyńska-Krzysik |first8=Anna |last9=Zaleski |first9=Piotr |last10=Płucienniczak |first10=Andrzej |date=2019-05-01 |title=Expression and purification of recombinant human insulin from E. coli 20 strain |journal=Protein Expression and Purification |volume=157 |pages=63–69 |doi=10.1016/j.pep.2019.02.002 |pmid=30735706 |doi-access=free }}</ref>