Editing DNA sequencer (section)

==Comparison==
[[List of DNA sequencers|Current offerings in DNA sequencing technology]] show a dominant player: [[Illumina, Inc.|Illumina]] (December 2019), followed by [[Pacific Biosciences|PacBio]], [[BGI Group|MGI]] and [[Oxford Nanopore Technologies|Oxford Nanopore]].
{| class="wikitable"
|+ Comparing metrics and performance of next-generation DNA sequencers.<ref name="shendure">{{cite journal | last1 = Shendure | first1 = J. | last2 = Ji | first2 = H.  | year = 2008 | title = Next-generation DNA sequencing | journal = Nat. Biotechnol. | volume = 26 | issue = 10| pages = 1135–1145 | doi=10.1038/nbt1486 | pmid=18846087| s2cid = 6384349 }}</ref>
|-
! Sequencer
! '''Ion Torrent PGM'''<ref name="a tale of three">{{Cite journal |last1=Quail |first1=Michael A. |last2=Smith |first2=Miriam |last3=Coupland |first3=Paul |last4=Otto |first4=Thomas D. |last5=Harris |first5=Simon R. |last6=Connor |first6=Thomas R. |last7=Bertoni |first7=Anna |last8=Swerdlow |first8=Harold P. |last9=Gu |first9=Yong |date=2012-07-24 |title=A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers |journal=BMC Genomics |volume=13 |issue=1 |pages=341 |doi=10.1186/1471-2164-13-341 |pmid=22827831 |pmc=3431227 |issn=1471-2164 |doi-access=free }}</ref><ref name="karow agbt">{{Cite web |last=Karow |first=Julia |date=2010-03-02 |title=Ion Torrent Systems Presents $50,000 Electronic Sequencer at AGBT |url=https://www.genomeweb.com/sequencing/ion-torrent-systems-presents-50000-electronic-sequencer-agbt |website=GenomeWeb |language=en}}</ref><ref name=PGM>{{Cite web |url=http://www.iontorrent.com/products-ion-pgm/ |title=Ion PGM - Ion Torrent<!-- Bot generated title --> |access-date=2013-02-13 |archive-url=https://web.archive.org/web/20120920083447/http://www.iontorrent.com/products-ion-pgm |archive-date=2012-09-20 |url-status=dead }}</ref>
! '''454 GS FLX'''<ref name="Comparison Next-Gen Seq" />
! '''HiSeq 2000'''<ref name="a tale of three"/><ref name="Comparison Next-Gen Seq" />
! '''SOLiDv4'''<ref name="Comparison Next-Gen Seq" />
! '''PacBio'''<ref name="a tale of three"/><ref name=PacBio>{{Cite web|url=https://www.pacb.com/|title=Home - PacBio - Sequence with Confidence|website=PacBio}}</ref>
! '''Sanger 3730xl'''<ref name="Comparison Next-Gen Seq" />
! '''MGI DNBSEQ-G400'''<ref name="biorxiv-DNBSEQ-G400">{{Cite bioRxiv |biorxiv=10.1101/2020.07.02.185710 |first1=Xiaohuan |last1=Sun |first2=Jingjing |last2=Wang |title=Efficient and stable metabarcoding sequencing from DNBSEQ-G400 sequencer examined by large fungal community analysis |date=2020-07-03 |language=en |last3=Fang |first3=Chao |last4=Li |first4=Jiguang |last5=Han |first5=Mo |last6=Wei |first6=Xiaofang |last7=Zheng |first7=Haotian |last8=Luo |first8=Xiaoqing |last9=Gong |first9=Meihua |last10=Xiao |first10=Liang |last11=Hu |first11=Yuehua |last12=Song |first12=Zewei}}</ref>
|-
| Manufacturer
| Ion Torrent (Life Technologies)
| 454 Life Sciences (Roche)
| Illumina
| Applied Biosystems (Life Technologies)
| Pacific Biosciences
| Applied Biosystems (Life Technologies)
| MGI
|-
| Sequencing Chemistry
| Ion semiconductor sequencing
| [[Pyrosequencing]]
| Polymerase-based sequence-by-synthesis
| [[Sequencing by ligation|Ligation-based sequencing]]
| Phospholinked fluorescent nucleotides
| Dideoxy chain termination
| Polymerase-based sequence-by-synthesis
|-
| Amplification approach
| Emulsion PCR
| Emulsion PCR
| Bridge amplification
| Emulsion PCR
| Single-molecule; no amplification
| PCR
| DNA nanoball (DNB) generation
|-
| Data output per run
| 100-200 Mb
| 0.7 Gb
| 600 Gb
| 120 Gb
| 0.5 - 1.0 Gb
| 1.9~84 Kb
| 1440 Gb / 1500-1800M reads
|-
| Accuracy
| 99%
| 99.9%
| 99.9%
| 99.94%
| 88.0% (>99.9999% CCS or HGAP)
| 99.999%
| 99.90%
|-
| Time per run
| 2 hours
| 24 hours
| 3–10 days
| 7–14 days
| 2–4 hours
| 20 minutes - 3 hours
| 3–5 days
|-
| Read length
| 200-400 bp
| 700 bp
| 100x100 bp paired end
| 50x50 bp paired end
| 14,000 bp ([[N50 statistic|N50]])
| 400-900 bp
| 100/150/200 bp paired end
|-
| Cost per run
| US$350
| US$7,000
| US$6,000 (30x human genome)
| US$4,000
| $125–300 USD
| US$4 (single read/reaction)
| N/A
|-
| Cost per Mb
| US$1.00
| US$10
| US$0.07
| US$0.13
| $0.13 - US$0.60
| US$2400
| $0.007
|-
| Cost per instrument
| US$80,000
| US$500,000
| US$690,000
| US$495,000
| US$695,000
| US$95,000
| N/A
|}